ANTIBACTERIAL ACTIVITY TEST OF BANANA PEEL ESSENTIAL OIL AGAINST ESCHERICHIA COLI (E. COLI) BACTERIA IN BIOCHEMISTRY LABORATORYUM
Keywords:
antibacterial activity banana peel essential oil Biochemistry
Abstract
A study on the antibacterial activity of essential oil from banana peel was conducted. The research was conducted in a span of two months starting from sample collection, sample distillation process, and testing the antibacterial activity of essential oil. This study was conducted to determine the antibacterial activity of banana peel essential oil as a natural antibacterial against Escherichia coli (E. coli) bacteria considering the resistance of synthetic antibacterials to pathogenic bacteria. Isolation of essential oil in banana peel using steam distillation method and antibacterial activity test using disc paper method. The distilled essential oil was purified using anhydrous sodium sulphate (Na2SO4) to separate the oil from water and other impurities without affecting the purity of the essential oil. The results of the antibacterial activity test of banana peel essential oil against Escherichia coli (E. coli) bacteria with a concentration of 50% obtained an inhibition zone of 10.5 mm and at a concentration of 25% of 9.1 mm including the medium category. More than 50% of banana peel essential oil is thought to be more effective because at that concentration level, antibacterial compounds that have potential as antibacterial agents have reached sufficient levels.
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References
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Anggraini, R., Jayuska, A., & Alimuddin, A. H. (2018). Lada Hitam (Piper Nigrum L.) Asal Sajingan Kalimantan Barat. Jurnal Kimia Khatulistiwa, 7(4), 124–125.
Aptari, Z. (2021). Uji Efektivitas Pertumbuhan Lactobacillus Casei Pada Media Umbi Ganyong Merah Dan Ganyong Putih. Jurnal Bioshell, 10(1), 32–39. Https://Doi.Org/10.36835/Bio.V10i1.991
Aryani, F., Noorcahyati, & Arbainsyah. (2020). Pengenalan Atsiri (Melaleuca Cajuputi). Jurusan Teknologi Pertanian Politeknik Pertanian Negeri Samarinda, 1–38.
Bahri, S., Ginting, Z., Vanesa, S., & Za, N. (2021). Formulasi Sediaan Gel Minyak Atsiri Tanaman Nilam (Pogostemon Cablin Benth) Sebagai Antiseptik Tangan (Hand Sanitizer). Jurnal Teknologi Kimia Unimal, 8(1), 87. Https://Doi.Org/10.29103/Jtku.V10i1.4179
Ecevit, K., Barros, A. A., Silva, J. M., & Reis, R. L. (2022). Preventing Microbial Infections With Natural Phenolic Compounds. Future Pharmacology, 2(4), 460–498. Https://Doi.Org/10.3390/Futurepharmacol2040030
Elangovan, S., & Mudgil, P. (2023). Antibacterial Properties Of Eucalyptus Globulus Essential Oil Against Mrsa: A Systematic Review. Antibiotics, 12(3), 474. Https://Doi.Org/10.3390/Antibiotics12030474
Fajrina, R. F. N., Rahayu, I. G., Wahyuni, Y., & Rahmat, M. (2019). Aktivitas Antibakteri Ekstrak Kulit Pisang Ambon (Musa Acuminata Colla) Terhadap Staphylococcus Aureus Secara in-Vitro. Jurnal Riset Kesehatan Poltekkes Depkes Bandung, 11(1), 230–235. https://doi.org/10.34011/juriskesbdg.v11i1.744
Fitriana, Y. A. N., Fatimah, V. A. N., & Fitri, A. S. (2020). Aktivitas Anti Bakteri Daun Sirih: Uji Ekstrak Khm (Kadar Hambat Minimum) Dan Kbm (Kadar Bakterisidal Minimum). Sainteks, 16(2), 101–108. Https://Doi.Org/10.30595/St.V16i2.7126
Hanifah, A. (2018). Potensi Minyak Atsiri Dalam Menghambat Pertumbuhan Isolat Bakteri Yang Ditemukan Di Candi Borobudur. Jurnal Konservasi Cagar Budaya, 12(2), 11–22. Https://Doi.Org/10.33374/Jurnalkonservasicagarbudaya.V12i2.185
Herda Ariyani, Muhammad Nazemi, Hamidah, M. K. (2018). Uji Efektivitas Antibakteri Ekstrak Kulit Limau Kuit ( Cytrus Hystrix Dc ) Terhadap Beberapa Bakteri ( The Effectiveness Of Antibacterial The Citrus Lime Peel Extract ( Citrus Hystrix Dc ) Of Some Bacteria ). 2(1), 136–141.
Ida Ayu Raka Astiti Asih, Wiwik Susanah Rita, I Gusti Bagus Teguh Ananta, N. K. D. M. S. W. (2018). Aktivitas Antibakteri Ekstrak Kulit Pisang ( Musa Sp.) Terhadap Escherichiacoli Dan Staphylococcus Aureus Serta Identifikasi Golongan Senyawa Aktifnya. Cakra Kimia, 6(Mic), 56–63.
Juariah, S. (2021). Media Alternatif Pertumbuhan Staphylococcus Aureus Dari Biji Durian (Durio Zibethinus Murr). Meditory : The Journal Of Medical Laboratory, 9(1), 19–25. Https://Doi.Org/10.33992/M.V9i1.1400
Nurjanah, G. S., Cahyadi, A. I., & Windria, S. (2020). Escherichia Coli Resistance To Various Kinds Of Antibiotics In Animals And Humans: A Literature Study. Indonesia Medicus Veterinus, 9(6), 970–983. Https://Doi.Org/10.19087/Imv.2020.9.6.970
Nurviana, V. (2018). Skrining Aktivitas Antibakteri Fraksi Ekstrak Etanol Kernel Biji Buah Limus (Mangifera Foetida Lour.) Terhadap Staphylococcus Aureusdan Escherichia Coli. Journal Of Pharmacopolium, 1(1), 37–43. Https://Doi.Org/10.36465/Jop.V1i1.394
Sari, D. K., Ibrahim, A. R., & Wancik, K. A. (2019). Designing biochemistry project with local materials to increased student’s creativity. Journal of Physics: Conference Series, 1166(1). https://doi.org/10.1088/1742-6596/1166/1/012007
Sari, D. K., Permanasari, A., & Supriyanti, F. M. T. (2017). Profile of students’ creative thinking skills on quantitative project-based protein testing using local materials. Jurnal Pendidikan IPA Indonesia, 6(1), 71–75. https://doi.org/10.15294/jpii.v6i1.9516
Sari, D. K., Wancik, K. A., Sufiana, J. M., & Haryani, M. E. (2021). Analysis of increasing creative thinking skills in the use of local materials in project qualitative biochemical analysis practicum. IOP Conference Series: Earth and Environmental Science, 1796(1). https://doi.org/10.1088/1742-6596/1796/1/012033
Sari, D. K., Wancik, K. A., & Ibrahim, A. R. (2019). Orbital : Jurnal Pendidikan Kimia. Jurnal Pendidikan Kimia, 3, 155–166.
Triastuti, A., Chabib, L., & Andiani, N. (2020). Pengembangan Hand Sanitizer Minyak Atsiri Daun Sirih: Profil Metabolit, Aktivitas Antibakteri, Dan Formulasi. Prosiding Seminar Nasional Hasil Penelitian Dan Pengabdian Masyarakat, (November), 43–55.
Wikantika, K. (2021). Pisang Indonesia.
Winda, F. R., Suparno, S., & Prasetyo, Z. K. (2023). Antibacterial Activity of Cinnamomum burmannii Extract Against Escherichia coli. Jurnal Penelitian Pendidikan IPA, 9(11), 9162–9170. https://doi.org/10.29303/jppipa.v9i11.4045
Published
2025-06-16
How to Cite
sariD. (2025, June 16). ANTIBACTERIAL ACTIVITY TEST OF BANANA PEEL ESSENTIAL OIL AGAINST ESCHERICHIA COLI (E. COLI) BACTERIA IN BIOCHEMISTRY LABORATORYUM. SPEKTRA: Jurnal Kajian Pendidikan Sains, 11(1), 113-123. https://doi.org/https://doi.org/10.32699/spektra.v11i1.8172
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Article
